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IFN‐γ overcomes low responsiveness of myeloid dendritic cells to CpG DNA
Author(s) -
Uchijima Masato,
Nagata Toshi,
Aoshi Taiki,
Koide Yukio
Publication year - 2005
Publication title -
immunology and cell biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0818-9641
DOI - 10.1111/j.1440-1711.2005.01307.x
Subject(s) - tlr9 , myeloid , cpg oligodeoxynucleotide , cpg site , secretion , stimulation , toll like receptor , dendritic cell , microbiology and biotechnology , function (biology) , toll like receptor 9 , receptor , immunology , biology , chemistry , innate immune system , gene expression , immune system , gene , neuroscience , dna methylation , endocrinology , genetics
Dendritic cells (DC) are professional APC that have an extraordinary capacity to prime naive T cells. It has been reported that human DC subsets express distinct toll‐like receptor (TLR), which influences their function. In mice, we observed that plasmocytoid DC (pDC) express a higher level of TLR9 compared with myeloid DC (mDC) cultured with GM‐CSF. However, we demonstrated that stimulation with IFN‐γ is capable of upregulating TLR9 expression in mDC to a level comparable with expression in pDC. Consistent with this observation, IL‐12 p40 and IL‐6 mRNA expression and IL‐12 p70 secretion in response to CpG‐oligodeoxynucleotides are enhanced in mDC pretreated with IFN‐γ compared with untreated cells. Therefore, TLR‐mediated responses of DC subsets may be influenced not only by signals delivered by pathogens but also by regulatory signals from cytokines such as IFN‐γ.