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Micromere Differentiation in the Sea Urchin Embryo: Expression of Primary Mesenchyme Cell Specific Antigen during Development
Author(s) -
SHIMIZU KEIKO,
NORO NOBUHIRO,
MATSUDA RYOICHI
Publication year - 1988
Publication title -
development, growth and differentiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.864
H-Index - 66
eISSN - 1440-169X
pISSN - 0012-1592
DOI - 10.1111/j.1440-169x.1988.00035.x
Subject(s) - mesenchyme , microbiology and biotechnology , embryo , sea urchin , ingression , biology , antigen , expression (computer science) , cellular differentiation , immunology , embryogenesis , computer science , gastrulation , genetics , gene , programming language
The temporal and spatial expression of antigen specific for primary mesenchyme cell (PMC) lineage cells during early development of the sea urchins Hemicentrotus pulcherrimus and Stronglyocentrotus nudus was studied with a monoclonal antibody (P4). P4 was produced by a hybridoma cell line prepared by fusion of myeloma cells and spleen cells from a mouse immunized with cultured spicule‐forming cells. Immunofluorescence studies demonstrated that P4 antibody reacted strongly with the surfaces of PMC's and spicule‐forming cells of both species. Immunoblot analysis showed that P4 antibody reacted with several proteins including those of 140–kDa, 120–kDa, 53‐kDa, 43–kDa, and 41–kDa in H. pulcherrimus and with those of 130–kDa, 110–kDa, 51–kDa, and 43–kDa in S. nudus . These proteins appeared sequentially after the hatching blastula stage. Tunicamycin inhibited the expressions of these P4 antigens as well as spicule formation. Two of the P4‐reactive antigens, the 140–kDa and 43–kDa proteins, in H. pulcherrimus were synthesized de novo and shown to be identical to micromere differentiation specific proteins. These results suggest that P4 binds to specific molecules that are important in spicule formation in developing sea urchin embryos.