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EFFECTS OF VOLUME DEPLETION AND NaHCO 3 LOADING ON THE EXPRESSION OF Na + /H + EXCHANGER ISOFORM 3, Na + : HCO COTRANSPORTER TYPE 1 AND NITRIC OXIDE SYNTHASE IN RAT KIDNEY
Author(s) -
Ma Seong Kwon,
Kang Joo Sam,
Bae Eun Hui,
Choi Chan,
Lee JongUn,
Kim Suhn Hee,
Choi Ki Chul,
Kim Soo Wan
Publication year - 2008
Publication title -
clinical and experimental pharmacology and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.752
H-Index - 103
eISSN - 1440-1681
pISSN - 0305-1870
DOI - 10.1111/j.1440-1681.2007.04837.x
Subject(s) - cotransporter , sodium–hydrogen antiporter , nitric oxide synthase , chemistry , nitric oxide , gene isoform , volume (thermodynamics) , endocrinology , medicine , biochemistry , sodium , biology , organic chemistry , physics , quantum mechanics , gene
SUMMARY1 The effects of volume depletion and NaHCO 3 loading on the expression of Na + /H + exchanger isoform 3 (NHE3), Na + : cotransporter type 1 (NBC1) and neuronal (n) and inducible (i) isoforms of nitric oxide synthase (NOS) were determined in rat kidney. 2 Adult male Sprague‐Dawley rats were used. Rats were divided into four groups: (i) euvolaemic (EC); (ii) hypovolaemic (HC); (iii) euvolaemia with NaHCO 3 loading (EB); and (iv) hypovolaemia with NaHCO 3 loading (HB). The expression of NHE3, NBC1, nNOS and iNOS proteins was determined in the cortex of the kidney by immunoblotting and immunohistochemistry. Tissue content of nitric oxide (NO) metabolites (NO x ) were also determined in the cortex using a colourimetric assay. 3 Compared with the EC group, the expression of NHE3 and NBC1 was increased in the HC group and decreased in the EB group. Comparing the EB and HB groups, the expression of NHE3 and NBC1 was higher in the latter group. The expression of NHE3 was decreased and that of NBC1 was increased in the HB group compared with the HC group. The NO x content and nNOS expression were decreased in the hypovolaemic (HC) and NaHCO 3 ‐loaded (EB and HB) rats. Moreover, the expression of iNOS was decreased in the HB group compared with the other groups. 4 An altered volume status and NaHCO 3 loading may affect the regulation of NHE3 and NBC1 in the kidney and the endogenous NO system may play a role in the observed effects.