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Single‐Layer Centrifugation Through Colloid Positively Modifies the Sperm Subpopulation Structure of Frozen–Thawed Stallion Spermatozoa
Author(s) -
García B Macías,
Fernández L González,
Morrell JM,
Ferrusola C Ortega,
Tapia JA,
Martínez H Rodriguez,
Peña FJ
Publication year - 2009
Publication title -
reproduction in domestic animals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.546
H-Index - 66
eISSN - 1439-0531
pISSN - 0936-6768
DOI - 10.1111/j.1439-0531.2008.01276.x
Subject(s) - sperm , semen , centrifugation , andrology , biology , colloid , chromatography , chemistry , medicine
Contents The present study attempted to select the subpopulation of stallion spermatozoa that best survived a conventional freezing and thawing procedure, using centrifugation of post‐thawed semen samples through a single layer of a glycidoxypropyltrimethoxysilane‐coated silica colloid with a species‐specific formulation (Androcoll‐E™). After freezing and thawing, four sperm subpopulations were identified, listed as FT1 to FT4. While subpopulations FT1 and FT2 were characterized by low sperm velocity, high velocities characterized the ones called FT3 and FT4. The single‐layer centrifugation (SLC)‐handled sperm sample was enriched in subpopulation FT3, reaching a proportion of 82.6% of the present spermatozoa, in contrast with the non‐filtered control post‐thawed semen, where this sperm subpopulation only accounted for 16.3% of the total. It is concluded that in the equine industry, the SLC is a practical, easy‐to‐perform approach to improve the quality of equine frozen–thawed semen samples.