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Effects of Exogenous Tumour Necrosis Factor‐α on the Secretory Function of the Bovine Reproductive Tract Depend on Tumour Necrosis Factor‐α Concentrations
Author(s) -
Skarzynski DJ,
Piotrowska KK,
Bah MM,
Korzekwa A,
WoclawekPotocka I,
Sawai K,
Okuda K
Publication year - 2009
Publication title -
reproduction in domestic animals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.546
H-Index - 66
eISSN - 1439-0531
pISSN - 0936-6768
DOI - 10.1111/j.1439-0531.2007.01016.x
Subject(s) - necrosis , tumor necrosis factor alpha , tumor necrosis factor α , function (biology) , reproductive tract , biology , cancer research , andrology , chemistry , medicine , endocrinology , microbiology and biotechnology
Contents The aim of study was to correlate tumour necrosis factor‐α (TNF) infused doses used with the TNF concentrations achieved and with the secretory function of both the ovary and the uterus in cows. We evaluated the concentrations of progesterone (P4), prostaglandin (PG)F 2α , PGE 2 nitric oxide (NO) and TNF in the jugular vein and vena cava caudalis as parameters of exogenous TNF action on the female reproductive tract. Aortae abdominalis of cows (n = 18) were infused with saline or two doses of TNF (luteolytic – 1 μg or luteotrophic – 10 μg). In the peripheral blood, 1 μg TNF concentrations achieved within the range of 30–45 pg/ml, and 10 μg TNF provoked a sharp increase in achieved concentrations at a range of 250–450 pg/mL). The TNF concentrations achieved in vena cava caudalis were five to six times higher than that in peripheral blood (p < 0.001). One microgram TNF increased PGF 2α and NO (p < 0.001) and decreased P4 (p < 0.05). The higher TNF dose stimulated P4 and PGE 2 (p < 0.01). TNF infusion at luteolytic dose achieved its concentrations at the physiological range previously observed in cows. Luteotrophic TNF dose achieved the concentrations in vena cava caudalis that are much higher than physiological level and were previously noted in pathological circumstances (i.e. mastitis , metritis ).