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Automation of DNA marker analysis for molecular breeding in crops: practical experience of a plant breeding company
Author(s) -
Dayteg C.,
Tuvesson S.,
Merker A.,
Jahoor A.,
KolodinskaBrantestam A.
Publication year - 2007
Publication title -
plant breeding
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.583
H-Index - 71
eISSN - 1439-0523
pISSN - 0179-9541
DOI - 10.1111/j.1439-0523.2007.01306.x
Subject(s) - canola , biology , automation , flexibility (engineering) , microbiology and biotechnology , throughput , marker assisted selection , polymerase chain reaction , selection (genetic algorithm) , biochemical engineering , computational biology , genetic marker , computer science , agronomy , engineering , genetics , gene , artificial intelligence , mathematics , wireless , mechanical engineering , telecommunications , statistics
Abstract In modern plant breeding, DNA marker analyses are of increasing importance and, as the methods become more widely adopted, the capacity for high‐throughput analyses at low cost is crucial for its practical use. Automation of the analysis processes is a way to meet these requirements. In order to achieve this, while keeping adequate flexibility in the analysis processes, Svalöf Weibull AB (SW) has developed a fully automated polymerase chain reaction system. It has been evaluated on barley and canola lines and is capable of analysing up to 2200 samples per day at a cost of 0,24 € per analysis for marker‐assisted selection and quality control of genetically modified organisms. A detailed description of this system is given, and improvements to the throughput and applications are discussed.