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Müeller–Hinton methylene blue media as an alternative to RPMI 1640 for determining the susceptibility of Cryptococcus neoformans and Cryptococcus gattii to posaconazole with Etest
Author(s) -
AlvaradoRamírez Eidi,
TorresRodríguez Josep M.,
Murciano Francisca,
Sellart Maite
Publication year - 2010
Publication title -
mycoses
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.13
H-Index - 69
eISSN - 1439-0507
pISSN - 0933-7407
DOI - 10.1111/j.1439-0507.2008.01678.x
Subject(s) - etest , cryptococcus neoformans , posaconazole , methylene blue , microbiology and biotechnology , cryptococcus , cryptococcosis , medicine , chemistry , biology , antifungal , amphotericin b , antibiotics , organic chemistry , photocatalysis , catalysis
Summary Mueller–Hinton modified agar (MH‐GMB) was compared with RPMI + 2% glucose–agar to determine the MICs of 80 isolates of Cryptococcus neoformans and C. gattii to posaconazole with Etest. MH‐GMB minimised trailing and agreement between both media was 94%. Agreement of M27‐A2 microbroth reference method was 98% with RPMI and 94% with MB‐GMB.