Premium
An RNA Probe for the Detection of Grapevine virus A
Author(s) -
Komínek Petr,
KomínkováBryxiová Marcela,
Jandurová Olga Mercedes,
Holleinová Věra
Publication year - 2008
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1111/j.1439-0434.2008.01446.x
Subject(s) - biology , dot blot , rna , plant virus , virology , virus , vitis vinifera , vine , microbiology and biotechnology , rna extraction , hybridization probe , botany , dna , genetics , gene
A specific dot‐blot hybridization assay was developed using an in vitro ‐transcribed non‐radioactive RNA probe for the routine detection of Grapevine virus A (GVA) for use as an alternative diagnostic tool for large‐scale surveys. GVA was detected in RNA isolated from infected vine plants, plant extracts and tissue prints using this probe however no reaction was observed with healthy plants of genus Vitis .