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A Rapid and Sensitive Method for Direct Detection of Erwinia amylovora in Symptomatic and Asymptomatic Plant Tissues by Polymerase Chain Reaction
Author(s) -
Stöger A.,
Schaffer J.,
Ruppitsch W.
Publication year - 2006
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1111/j.1439-0434.2006.01130.x
Subject(s) - biology , erwinia , polymerase chain reaction , asymptomatic , microbiology and biotechnology , real time polymerase chain reaction , virology , bacteria , genetics , gene , pathology , medicine
Abstract Fire blight is a destructive disease of apple and pear trees and other rosaceous plants caused by the Gram‐negative bacterium Erwinia amylovora . Several molecular detection procedures described for this plant pathogen are either too time consuming, too insensitive or impractical when handling a large number of samples routinely. Here, we describe a modified protocol of the REDExtract‐N‐Amp TM Plant polymerase chain reaction kit for the detection of E. amylovora in planta and demonstrate that it provides simplicity, high sensitivity, speed and high throughput potential. A total of 951 samples collected in Austria were tested with the modified protocol and the standard protocol of our laboratory to validate the accuracy of this detection method.