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Occurrence of Colombian datura virus in the Terrestrial Orchid, Spiranthes cernua
Author(s) -
Fry C. R.,
Zimmerman M. T.,
Scott S. W.
Publication year - 2004
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1111/j.1439-0434.2004.00827.x
Subject(s) - biology , ornamental plant , orchidaceae , botany , virus , polymerase chain reaction , virology , genetics , gene
Colombian datura virus was detected in the terrestrial orchid Spiranthes cernua using a combination of enzyme‐linked immunosorbent assay (ELISA) and polymerase chain reaction (PCR). The PCR strategy that we used to detect and confirm the identity of this virus has subsequently been used as a generalized procedure to confirm the identity of other potyviruses in ornamental species. This is the first report of this virus in an orchid and only the second report of a virus in this species.
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