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Differentiation of Closely Related Globodera pallida (Stone) Populations by Means of DNA Restriction Fragment Length Polymorphisms (RFLPs)
Author(s) -
Schnick D.,
Rumpenhorst H. J.,
Burgermeister W.
Publication year - 1990
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1111/j.1439-0434.1990.tb01160.x
Subject(s) - biology , restriction fragment length polymorphism , globodera pallida , dendrogram , genetics , population , genomic dna , genetic distance , dna , genetic variation , polymerase chain reaction , gene , genetic diversity , solanaceae , demography , sociology
Abstract A small genomic library from DNA of G. pallida population Delmsen (pathotype 3) was constructed. A number of clones were tested as hybridization probes with 21 G. pallida and 6 G. rostochiensis populations. The two species were easily distinguished by their hybridization patterns. A total of 32 RFLP markers were identified which permitted the differentiation of all G. pallida populations. By means of cluster analysis of the RFLP data the genetic distances between the 21, G. pallida populations were calculated and a dendrogram was constructed. The arrangement of the 21 G. pallida populations in the dendrogram suggests a partial correlation between genetic distance and virulence according to biological pathotyping.