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Isolation of Atlantic salmon ( Salmo salar L.) cytochrome c oxidase subunit II gene ( coxII )
Author(s) -
Hardiman G.,
Wolff J.,
Peden J.,
Gan F.
Publication year - 1994
Publication title -
journal of applied ichthyology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.392
H-Index - 62
eISSN - 1439-0426
pISSN - 0175-8659
DOI - 10.1111/j.1439-0426.1994.tb00143.x
Subject(s) - biology , salmo , complementary dna , microbiology and biotechnology , gene , cdna library , coding region , southern blot , cytochrome c oxidase , genetics , fishery , mitochondrion , fish <actinopterygii>
Summary Screening an Atlantic salmon ( Salmo salar L.) salmon kidney cDNA library with total cDNA led to the isolation of a clone which hybridised strongly to both salmon kidney and spleen total cDNA probes. Analysis of the sequence identified the cDNA as the gene coding for subunit II of cytochrorne c oxidase ( coxII ). Northern blot studies revealed a transcript of approximately 700 bp in liver poly A+ mRNA. The DNA sequence of the Atlantic salmon coxII gene employs the mammalian mitochondrial genetic code and is strongly conserved when compared to other species.

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