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Differential transcription of the bovine AMPD3 gene in single blastocysts cultured under various oxygen tensions
Author(s) -
Yuan Y. Q.,
Peelman L. J.,
Williams J. L.,
Van Zeveren A.,
Kruif A.,
Law A.,
Van Soom A.
Publication year - 2004
Publication title -
journal of animal breeding and genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.689
H-Index - 51
eISSN - 1439-0388
pISSN - 0931-2668
DOI - 10.1111/j.1439-0388.2004.00469.x
Subject(s) - embryo , biology , gene , gene expression , microbiology and biotechnology , in vitro , differential display , chromosome , genetics
Summary Studying gene expression during the early embryonic stages of in vitro culture is essential and of special interest, not only to gain insights in embryo development but also to find candidate genes that can be used as markers to assess embryo quality. Using differential display RT‐PCR expression of AMPD3 coding for AMP deaminase 3 was found in bovine blastocysts cultured in vitro only under 2% but not under 5 and 20% oxygen tensions. Mapping of this gene to bovine chromosome 15 confirmed its identity. The differential expression of bovine AMPD3 was confirmed by RT‐PCR with specific primers.