Open AccessCloning and Characterization of the Oxytricha Granulifera Chaperonin Containing Tailless Complex Polypeptide 1 γ Gene
Author(s) -
Palmedo Gabriele,
Ammermann Dieter
Publication year - 1997
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1997.00877.x
Subject(s) - biology , gene , microbiology and biotechnology , primer extension , coding region , chaperonin , northern blot , cloning (programming) , gene expression , amino acid , messenger rna , southern blot , tetrahymena , heat shock protein , genetics , computer science , programming language
Here we report the cloning and characterization of the Oxytricha granulifera CCTγ (chaperonin containing tailless complex polypeptide 1) gene encoding a protein that consists of 559 amino acids. The derived amino acid sequence shares 68% identity with Tetrahymena pyriformis CCTγ and 58% identity with Mus musculus CCTγ. The O. granulifera CCTγ gene is located on a 2.07‐kbp macronuclear gene‐sized piece. Its transcription initiation site was determined by primer extension analysis. The expression of the CCTγ gene was investigated by northern blot hybridization of the coding region with exponentially growing cells, heat shocked cells, and cells treated with 50 μM CdCl 2 . After heat shock, a decrease in the amount of the transcript compared to that of exponentially growing cells could be shown. In contrast, higher amounts of the transcript could be detected after treatment with CdCl 2 .