Open AccessPreparation and characterization of pig plasma and platelet gelsolins
Author(s) -
WEEDS Alan G.,
GOOCH John,
POPE Brian,
HARRIS Harriet E.
Publication year - 1986
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1986.tb10125.x
Subject(s) - gelsolin , affinity chromatography , chemistry , sepharose , biochemistry , cleavage (geology) , ethylene glycol , size exclusion chromatography , chromatography , calcium , egta , cellulose , platelet , actin , biology , enzyme , organic chemistry , fracture (geology) , immunology , paleontology
Pig plasma gelsolin has been prepared by a revised method involving poly(ethylene glycol) precipitation, chromatography on CM‐cellulose and affinity chromatography on actin‐Sepharose. Pig platelet gelsolin has been prepared by chromatography on DEAE‐cellulose and actin‐Sepharose. Partial chemical and proteolytic cleavage shows that the two proteins are closely related in their fragmentation patterns. The amino acid sequences are identical at the N‐terminus of the platelet protein, but the plasma protein has an additional nine residues on the N‐terminal side of the common sequence. Calcium binding studies show that the plasma protein has similar calcium binding properties to both macrophage and platelet gelsolins.