Open AccessRelevance of the lariat model to the splicing of the H‐2 gene family
Author(s) -
BOUGUELERET Lydie,
CLAVERIE JeanMichel
Publication year - 1986
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1986.tb09968.x
Subject(s) - intron , rna splicing , gene , genetics , group ii intron , biology , alternative splicing , branching (polymer chemistry) , computational biology , exon , chemistry , rna , organic chemistry
We examined the splicing of the H‐2 gene family, taking the H‐2K d as a prototype, in the framework of the lariat model. We mainly investigated the mechanism described by Konarska et al. [ Nature (Lond.) 313 , 552–557 (1985)] who propose a direct interaction between the 5′ splicing site and the branching region. We also checked each of the H‐2 introns for the presence of patterns resembling the published consensus for the branching region. The known splicing events in the H‐2 gene family are not always consistent with the current models, and our results indicate that slightly different mechanisms govern the splicing of different introns. A tentative explanation of the alternative splicing of the first and last intron, previously described, is given. The removal of the large third intron is not easily rationalized unless new rules for an additional multistep processing are postulated.