Enzymes Synthesizing and Hydrolyzing Murein in Escherichia coli

Envelopes from regions of the cell which in vivo show very little, if any, murein synthesis were isolated using the minicell‐producing strain P678‐54. Envelopes from minicells, representing in fact cell ends, were able to synthesize murein and to carry out transpeptidation in vitro; also all four murein hydrolase activities tested, carboxypeptidase, endopeptidase, amidase and transglycosylase, were found to be present. The specific activities of the murein synthesizing and degrading enzymes in envelopes derived from cell poles and from actively growing cells were similar. The topological distribution of murein‐synthesizing enzymes and of murein hydrolases over the cell envelope is discussed.