Open AccessSites of Action of Fusidic Acid in Eukaryotes
Author(s) -
MAZUMDER Rajarshi
Publication year - 1975
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1975.tb02404.x
Subject(s) - fusidic acid , gtpase , ribosome , biology , artemia salina , gtp' , microbiology and biotechnology , biochemistry , chemistry , bacteria , rna , enzyme , genetics , staphylococcus aureus , gene , organic chemistry , toxicity
1 A ribosome‐independent GTPase activity has been isolated from the high‐speed supernatant fraction of Artemia salina embryos, and some of its properties have been studied. This activity is inhibited by fusidic acid, an antibiotic generally thought to inhibit only EF‐2 in eukaryotes. However, several lines of evidence indicate that the GTPase activity, described here, is distinct from EF‐2. The results suggest, therefore, that the inhibitory effect of fusidic acid in eukaryotic systems is not restricted to EF‐2 (and ribosome)‐dependent functions only. 2 The results of other experiments have revealed that, despite its ability to inhibit the GTPase activity mentioned above, fusidic acid is not a non‐specific inhibitor of all ribosome‐independent GTPase and ATPase activities present in eukaryotic cells.