Open AccessThe Kinetics of Renaturation of 5‐S RNA from Escherichia coli in the Presence of Mg 2+ Ions
Author(s) -
Richards Edward G.,
Lecanidou Rena,
Geroch Mary E.
Publication year - 1973
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1973.tb02755.x
Subject(s) - kinetics , chemistry , escherichia coli , absorbance , arrhenius equation , rna , activation energy , ion , yield (engineering) , electrophoresis , crystallography , analytical chemistry (journal) , chromatography , biochemistry , thermodynamics , organic chemistry , physics , quantum mechanics , gene
The kinetics of renaturation of denatured 5‐S RNA from Escherichia coli have been studied by estimating the composition of reaction mixtures from absorbance profiles of stained zones containing the two forms separated by polyacrylamide‐gel electrophoresis. The kinetics are first order and proceed to completion irrespective of temperature or Mg 2+ ion concentration, but they depend strongly on temperature. Linear Arrhenius plots yield an activation energy of 65 kcal/mol RNA. It is suggested that about nine base pairs of the denatured form dissociate and about the same number reform in a different configuration in the renatured conformer.
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