Open AccessIncorporation of 3‐Methyl‐2‐benzothiazolone Hydrazone into the Anticodon Loop of tRNA Phe from Yeast
Author(s) -
Freist Wolfgang,
Maelicke Alfred,
Sprinzl Mathias,
Cramer Friedrich
Publication year - 1972
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1972.tb02521.x
Subject(s) - hydrazone , transfer rna , chemistry , ribose , nucleotide , yeast , nucleoside , oligomer , covalent bond , stereochemistry , fluorescence , biochemistry , rna , organic chemistry , enzyme , physics , quantum mechanics , gene
The incorporation of 3‐methyl‐2‐benzothiazolone hydrazone into the anticodon loop of tRNA Phe from yeast after excision of the base Y has been studied with respect to concentration, pH and temperature. Model reactions were performed at the monomer level with ribose and ribose 5‐phosphate and at the oligomer level with a tRNA Phe anticodon‐arm dodecanucleotide from which the base Y had been excised. The reaction is easily followed by ultraviolet spectroscopy. The reaction product exhibits weak fluorescence. The covalent attachment of 3‐methyl‐2‐benzothiazolone hydrazone to the nucleotide chain is stable. As shown by nucleoside analysis no side reactions occur and the incorporation of 3‐methyl‐2‐benzothiazolone hydrazone into tRNA is quantitative. After modification tRNA Phe is fully chargeable.