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Duffy antigen expression on reticulocytes does not alter following blood loss in an autologous donation model
Author(s) -
Woolley I. J.,
Brown C. M. S.,
Hutchinson P.,
Turkou V.,
Visvanathan K.,
Wood E. M.
Publication year - 2009
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/j.1423-0410.2009.01203.x
Subject(s) - reticulocyte , antigen , immunology , medicine , reticulocytosis , blood donor , andrology , whole blood , antibody , donation , blood donations , flow cytometry , biology , biochemistry , messenger rna , anemia , gene , economics , economic growth
Background The Duffy blood group (Fy) antigen functions as the receptor whereby the malarial parasite Plasmodium vivax invades reticulocytes. In this study, we evaluated an autologous blood donation model to measure Fy expression during the anticipated response to blood loss. Aims This study aims to examine Fy expression following anticipated reticulocytosis in response to blood loss from autologous whole blood donation. Method Subjects were healthy blood donors presenting for planned collection of two or three autologous units. Whole blood (450 ml ± 10%) was collected and processed. Blood samples for Fy testing were obtained from the donations. These were assayed by flow cytometry by measuring binding of a phycoerythrin‐labelled anti‐Fy6 antibody and compared against reticulocyte numbers. Reticulocyte numbers were measured using thiazole orange. Results were compared from baseline (first donation) with samples at second and, if available, third, donations. Phenotyping for Fy a and b antigens was performed. Results Reticulocytes increased by a mean of 37% over baseline [0·93% (range 0·31–1·93) to 1·23% (0·32–3·51%)] following donation of two ( n = 32) or three ( n = 9) autologous whole blood units. Absolute reticulocyte count remained low. Mean and median Fy expression on mature red blood cells and reticulocytes did not change from baseline levels despite individual variation. No apparent relationship to serologically determined Fy a and/or b antigen status was present. Conclusion Baseline expression of Fy antigen on mature red blood cells and reticulocytes is quite variable between individuals, but appears not to be greatly affected by mild to moderate reticulocytosis following blood loss in an autologous blood donation model.