An Immunoblotting Technique for Direct Visualization of Chido and Rodgers Reactivity on C4 Variants after Electrophoresis | Zendy

Sklarin Peter M. | Zendy; Awdeh Zuheir L. | Zendy; Alper Chester A. | Zendy

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An Immunoblotting Technique for Direct Visualization of Chido and Rodgers Reactivity on C4 Variants after Electrophoresis

Author(s) -

Sklarin Peter M.,

Awdeh Zuheir L.,

Alper Chester A.

Publication year - 1988

Publication title -

vox sanguinis

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.68

H-Index - 83

eISSN - 1423-0410

pISSN - 0042-9007

DOI - 10.1111/j.1423-0410.1988.tb01611.x

Subject(s) - nitrocellulose , antiserum , electrophoresis , gel electrophoresis , enzyme , membrane , chemistry , microbiology and biotechnology , antigen , biology , biochemistry , immunology

. An immunoblotting technique allows direct visualization of Chido and Rodgers antigenic determinants on intact C4 proteins. C4 molecules separated by electrophoresis are selectively transferred to nitrocellulose membranes saturated with goat antiserum to human C4. The membranes are then incubated in alloanti‐Chido or anti‐Rodgers followed by enzyme‐conjugated goat antihuman IgG. Molecules with Chido or Rodgers reactivity are visualized by incubation with an indicator substrate for the bound enzyme.

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