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A Coated‐Tube Radioimmunoassay (FRC‐RIA) for Hepatitis B Surface Antigen
Author(s) -
Koistinen Vesa
Publication year - 1978
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1111/j.1423-0410.1978.tb02884.x
Subject(s) - radioimmunoassay , chromatography , incubation , antigen , antibody , reagent , chemistry , polystyrene , immunology , medicine , biochemistry , organic chemistry , polymer
Abstract. A sandwich‐type solid phase radioimmunoassay, FRC‐RIA (Finnish Red Cross‐RIA) for hepatitis B surface antigen (HB s Ag) is described. Polystyrene test tubes coated with sheep anti‐HB s γ‐globulin serve as the solid phase. The technique is specific and has a sensitivity of about 1 ng/ml for the subtypes ad and ay of HB s Ag when corresponding subtype‐specific antibodies are used as reagents. The total incubation time can be shortened from over 20 h to 3 h, with almost equal sensitivity, when the incubation temperature is raised from room temperature to 45°C. The test is economical in reagents and is applicable to routine use.