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In Vivo Induced Malignant Hyperthermia in Pigs II. Metabolism of Skeletal Muscle Mitochondria
Author(s) -
Ruitenbeek W.,
Verburg M. P.,
Janssen A. J. M.,
Stadhouders A. M.,
Sengers R. C. A.
Publication year - 1984
Publication title -
acta anaesthesiologica scandinavica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.738
H-Index - 107
eISSN - 1399-6576
pISSN - 0001-5172
DOI - 10.1111/j.1399-6576.1984.tb02002.x
Subject(s) - mitochondrion , in vivo , malignant hyperthermia , oxidative phosphorylation , skeletal muscle , creatine , medicine , creatine kinase , phosphate , halothane , in vitro , metabolism , glycolysis , endocrinology , biochemistry , biology , anesthesia , microbiology and biotechnology
The biochemical characteristics of skeletal muscle mitochondria of malignant hyperthermia (MH) susceptible Dutch Landrace pigs have been investigated before and during an MH attack, induced in vivo by halothane plus succinylcholine. The muscle homogenates have a decreased capacity to synthesize ATP and creatine phosphate during the MH period. Muscle mitochondria prepared from susceptible pigs in an MH period consume less oxygen than do mitochondria isolated before the attack, or mitochondria from control pigs during the challenge. The oxidative phosphorylation is not uncoupled during the critical period. The production of CO2 indicates that the in vitro measured capacity of the MH muscle mitochondria correctly reflects the in vivo condition during the MH attack. The restricted synthesis may be caused by a factor, finding expression in the mitochondria themselves, and obtained or activated during the MH attack.