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Endoribonuclease of Vicia faba ssp. minor radicles, and enhancement of its activity by chilling stress or abscisic acid
Author(s) -
Kazmierczak Andrzej,
Knypl J. Stan
Publication year - 1994
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1994.tb03011.x
Subject(s) - abscisic acid , vicia faba , endoribonuclease , fluridone , radicle , botany , rnase p , biochemistry , vicia , chemistry , biology , germination , rna , gene
Endoribonuclease in roots of 3‐day‐old seedlings of Vicia faba L. ssp, minor is a citrate activated glycoprotein of 35 kDa, with pH and temperature optima of 6.0 and 50°C. The same holds for endoribonuclease from seedlings treated with abscisic acid or exposed to a chilling stress of ‐3°C for 24 h, except that the temperature optimum was decreased to 40°C. The enzyme(s) preferentially hydrolysed poly (A) and poly (U). RNase activity in the radicles was enhanced by chilling stress or abscisic acid. ABA did not potentiate the effect brought about by chilling but slowed down the decrease in RNase activity in chill‐stressed seedlings upon transfer to 25°C. Both factors modified the pattern of the isoelectric‐points of the molecular forms of RNase.