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Induction of embryos in pollen cultures of Nicotiana sylvestris
Author(s) -
Rashid A.
Publication year - 1982
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1982.tb00329.x
Subject(s) - pollen , sucrose , biology , botany , nicotiana , embryo , isolation (microbiology) , solanaceae , microbiology and biotechnology , biochemistry , gene
Induction of embryos in pollen cultures was possible when young buds from plants induced to flower at 15°C were given a cold treatment at 10°C for 15 days. Following selective isolation of embryogenic pollen the cultures raised were given cold treatment at 10°C for 12 or more days. In such cultures the embryos appeared at a frequency of 1.2% of the cultured pollen on mineral‐sucrose medium at pH 6.8.