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Release of nucleotide‐cleaving acid phosphatase from carrot cells grown in suspension culture
Author(s) -
Ciarrocchi G.,
Cella R.,
Nielsen E.
Publication year - 1981
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1981.tb04515.x
Subject(s) - daucus carota , ribonucleoside , suspension culture , biochemistry , nucleotide , chemistry , phosphate , phosphatase , enzyme , acid phosphatase , cell culture , dna , biology , rna , botany , genetics , gene
Carrot ( Daucus carota L. cv. Lunga di Amsterdam) cells grown in suspension culture release into the culture medium a phosphatase capable of converting deoxyribo‐ as well as ribonucleoside triphosphates into nucleosides. The enzyme activity requires acidic pH and allows a prompt utilization of phosphorylated DNA precursors as measured by tritiated dTTP incorporation. Such utilization is partially inhibited by inorganic phosphate and completely inhibited by ATP.