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The Relationship between Chlorophyllide Accumulation, the Amount of Protochlorophyllide 636 and Protochlorophyllide 650 in Dark Grown Wheat Leaves Treated with δ‐Aminolevulinic Acid
Author(s) -
SUNDQVIST CHRISTER
Publication year - 1973
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1973.tb08590.x
Subject(s) - protochlorophyllide , chlorophyll , chemistry , transformation (genetics) , biochemistry , biosynthesis , enzyme , organic chemistry , gene
Abstract The influence of different amounts of protochlorophyl‐lide 636 and protochlorophyllide 350 on light Induced chloro‐phyllide formation was. studied in wheat leaves treated with δ‐aminolevulinic acid. The phototransformation of proto‐chlorophyllide was performed with weak red light. This transformation is unaffected by the δ‐aminolevulinic acid treatment, whilst the accumulation of chlorophyllide, both the rate and the amount, is greatly stimulated by moderate amounts of protochlorophyllide 636 . The presence of large amounts of protochlorophyllide 636 decreases the rate of chlorophyllide formation, but increases the final amount of chloro‐phyllide formed. A decreased level of protochlorophyllide 650 , obtained by treatment with N a N 3 , results in a decreased transformation rate. Inhibitors; of protein synthesis do not seem to influence the transformation of protochlorophyllide 636 to chlorophyllide, suggesting that no new synthesis of protein is required. The experimental results indicate that the final steps in chlorophyll biosynthesis are protochlorpnyllide 636 → protochlorophyllide 650 → chlorophyllide → chlorophyll.