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Cyclic Purine Mononucleotides: Induction of Gibberellin Biosynthesis in Barley Endosperm
Author(s) -
KESSLER BEZALEL,
KAPLAN BINA
Publication year - 1972
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1972.tb03638.x
Subject(s) - gibberellin , endosperm , gibberellic acid , purine , aleurone , biochemistry , biosynthesis , chemistry , amylase , dna synthesis , hordeum vulgare , de novo synthesis , dna , stereochemistry , biology , enzyme , botany , germination , poaceae
Abstract The de novo synthesis of α‐amylase in barley endosperm and isolated aleurone layers is induced by 3′,5′‐cyclic purine mononucleotides and gibberellic acid. The induction of α‐amylase by cyclic purine mononucleotides is prevented by 2,4‐DNP, inhibitors of RNA and protein syntheses, CCC, AMO‐1618 and phosfon. The induction of α‐amylase formation by 3′,5′‐cyclic purine mononucleotides, but not by gibberellic acid, is also blocked by inhibitors of DNA synthesis. Extracts from cyclic AMP‐treated endosperm halves exhibit a characteristic gibberellin‐like activity which is detectable within 12 hours from the addition of the cyclic AMP. On paper chromatograms this gibberellin‐like activity is located at the Rf typical for GA 3 . Its formation is prevented by inhibitors of DNA synthesis, CCC and AMO‐1618. Glucose inhibits the formation of α‐amylase induced by gibberellic acid. Glucose has no effect on the cAMP‐induced gibberellin biosynthesis. The evidence shows that the cyclic purine mononucleotides induce DNA synthesis, which results in gibberellin biosynthesis, which in turn activates the synthesis of α‐amylase.