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Recherches sur les enzymes catalysant la biosynthèse des acides phénoliques chez Quercus pedunculata . III. Formation séquentielle, à partir de la phénylalanine, des acides cinnamique, p‐coumarique et caféique, par des organites cellulaires isolés
Author(s) -
ALIBERT GILBERT,
RANJEVA RAOUL,
BOUDET ALAIN
Publication year - 1972
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1972.tb03608.x
Subject(s) - phenylalanine , caffeic acid , chemistry , biochemistry , enzyme , microsome , cinnamic acid , substrate (aquarium) , stereochemistry , amino acid , biology , antioxidant , ecology
Abstract The reactions leading to cinnamic acids from phenylalanine as only substrate were investigated in organelles from Quercus pedunculata Ehrh. roots. –“F 10 000′” fraction, including mitochondria and micro‐bodies, catalyses the first reaction, i.e. , cinnamate formation by deamination of phenylalanine. – Microsomal fraction catalyses all the steps from phenylalanine to caffeic acid via cinnamate and p‐coumarate. These results suggest that microsomes are the intracellular site of the cinnamic units synthesis. The enzymes involved in these reactions, associated in the same cellular compartment, does not form a multienzyme system. The formation of caffeic acid by isolated microsomes is demonstrated for the first time; the reaction may be realised by an enzyme different from phenolase. – The free phenolic acids are the metabolically active forms.