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Differentiation ex ovulo of Embryos and Plantlets in Stem Tissue Cultures of Tylophora indica
Author(s) -
Rao P. S.,
Narayanaswamy S.,
Benjamin Bathsion D.
Publication year - 1970
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1970.tb06401.x
Subject(s) - callus , biology , somatic embryogenesis , botany , embryo , tissue culture , shoot , somatic cell , in vitro , microbiology and biotechnology , embryogenesis , genetics , gene
Abstract Tylophora indica callus tissue repeatedly subcultured every month on a series of different nutrient media in sequence regenerated roots, shoots and typical, bipolar embryos in vitro from unorganized callus parenchyma. The embryo‐like structures developed from somatic cells grew into normal plantlets when isolated and cultured on appropriate milieu of nutrients and hormones bypassing the normal sexual method of reproduction. Likewise, free cells in suspension also passed through embryonic stages reminiscent of development from fertilized egg.