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Comparison of allergenic potentials of timothy ( Phleum pratense ) pollens from different pollen seasons collected in the Belgrade area
Author(s) -
Gavrović M. Dj.,
Trtić T.,
Vujčić Z.,
Petrović S.,
Jankov R. M.
Publication year - 1997
Publication title -
allergy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.363
H-Index - 173
eISSN - 1398-9995
pISSN - 0105-4538
DOI - 10.1111/j.1398-9995.1997.tb00977.x
Subject(s) - phleum , pollen , ragweed , ambrosia , allergen , biology , botany , immunoelectrophoresis , radioallergosorbent test , allergy , immunology , antibody
We investigated extracts of timothy grass pollen from four seasons (1989, 1990, 1991, and 1994) by protein content, SDS‐PAGE, immunoblot, RAST, RAST inhibition, and crossed immunoelectrophoresis. Extract of the pollen from 1991 showed the lowest yield in quantitative assays. SDS‐PAGE, crossed immunoelectrophoresis, RAST, and RAST inhibition expressed approximately comparable patterns for all extracts except that from 1991. Obviously, the quality of grass pollens, as shown for some ragweed ( Ambrosia elatior ) pollens depend on year of collection. Our findings are partially in agreement with some earlier examinations of the quality of timothy pollen from different pollen seasons.