Premium
Immunoblotting analysis of concanavalin A ‐isolated allergens of Candida albicans
Author(s) -
Savolainen J.,
Viander M.,
Einarsson R.,
Koivikko A.
Publication year - 1990
Publication title -
allergy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.363
H-Index - 173
eISSN - 1398-9995
pISSN - 0105-4538
DOI - 10.1111/j.1398-9995.1990.tb01082.x
Subject(s) - candida albicans , concanavalin a , corpus albicans , staining , mannose , immunoglobulin e , carbohydrate , blot , antibody , affinity chromatography , glycoprotein , chemistry , epitope , sepharose , biology , biochemistry , microbiology and biotechnology , immunology , in vitro , enzyme , gene , genetics
The carbohydrate‐containing Traction of Candida albicans was isolated from the crude extract with ConA Sepharose affinity chromatography and studied by IgE‐immuno‐blotting with individual and pooled sera from C. albicans ‐allergic subjects. In the ConA‐bound fraction there was a diffuse IgE binding in the high molecular weight area which also gave a carbohydrate stain (PAS). A distinct band corresponding to a molecular weight of 70 kD bound specific IgE antibodies. This glycoprotein, presumably a mannoprotein, gave a weak carbohydrate staining and a strong protein staining. further biochemical studies are needed to reveal the exact nature of the epitopes in the ConA‐bound mannose‐containing fraction of C. albicans.