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Production of Leukotrienes in Human Skin and Conjunctival Mucosa after Specific Allergen Challenge
Author(s) -
Bisgaard H.,
FordHutchinson A. W.,
Charleson S.,
Taudorf E.
Publication year - 1985
Publication title -
allergy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.363
H-Index - 173
eISSN - 1398-9995
pISSN - 0105-4538
DOI - 10.1111/j.1398-9995.1985.tb02680.x
Subject(s) - allergen , provocation test , medicine , leukotriene , stimulation , leukotriene c4 , in vivo , immunology , allergy , pathology , asthma , biology , alternative medicine , microbiology and biotechnology
The production of leukotrienes has been monitored in tear fluids from subjects following a conjunctival provocation test, and skin blister fluids following initiation of a Prausnitz‐Kustner reaction. In tear fluids elevated levels of leukotriene C 4 (LTC 4 )‐immunoreactive material were measured following allergen challenge as compared to control tear fluid obtained by mechanical or reflex stimulation. Analysis by high performance liquid chromatography indicated the presence of LTC 4 , LTD 4 and LTE 4 . In the skin, significantly elevated levels of LTC 4 ‐immunoreactive material were measured following allergen challenge in the Prausnitz‐Kustner reaction. HPLC analysis indicated the presence of both LTC 4 and LTD 4 . LTB 4 immunoreactive material was detected both in the tear fluid and the skin tissue fluid. However, no significant increase occurred in either tissue after the allergic reactions. These results indicate that the SRS‐A leukotrienes are released in vivo in man following allergen challenge, and indicate these mediators may be important in human allergic diseases.