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Effects of polyunsaturated fatty acids on isolated canine peripheral blood mononuclear cells and cytokine expression (IL‐4, IFN‐γ, TGF‐β) in healthy and atopic dogs
Author(s) -
Stehle Melanie E.,
Hanczaruk Matthias,
Schwarz Susanne C. N.,
Göbel Thomas W.,
Mueller Ralf S.
Publication year - 2010
Publication title -
veterinary dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.744
H-Index - 60
eISSN - 1365-3164
pISSN - 0959-4493
DOI - 10.1111/j.1365-3164.2009.00860.x
Subject(s) - peripheral blood mononuclear cell , polyunsaturated fatty acid , eicosapentaenoic acid , docosahexaenoic acid , concanavalin a , incubation , linoleic acid , cytokine , immunology , medicine , fatty acid , microbiology and biotechnology , biology , endocrinology , biochemistry , in vitro
Polyunsaturated fatty acids (PUFA) have been used to treat dogs with atopic dermatitis but the mechanism of action has not been well understood. The aim of this study was to evaluate the in vitro influence of PUFA on canine peripheral blood mononuclear cells (PBMC). PBMC isolated from eleven dogs with atopic dermatitis and eleven healthy control dogs were stimulated with concanavalin A and Dermatophagoides farinae extract in the presence of linoleic acid (LA), γ‐linolenic acid (GLA), α‐linolenic acid (ALA), eicosapentaenoic acid (EPA)/docosahexaenoic acid (DHA) and GLA/EPA/DHA. Subsequently, quantitative polymerase chain reaction (qPCR) for interferon (IFN)‐γ, interleukin (IL)‐4 and transforming growth factor (TGF)‐β m‐RNA was performed. In the presence of concanavalin A, only PBMC of healthy dogs showed a gradual reduction in proliferation index from incubation without PUFA to incubation with ALA, EPA/DHA and GLA/EPA/DHA, respectively. A similar reduction was seen in normal and in atopic dogs in the presence of D. farinae allergen after incubation with ALA, EPA/DHA and GLA/EPA/DHA. In both groups IL‐4 and IFN‐γ but not TGF‐β gene transcription was upregulated, when cells were incubated with D. farinae . Allergen‐induced upregulation was not influenced by incubation with PUFA. These findings suggest that PUFA are able to influence proliferation of peripheral blood mononuclear cells in healthy and atopic dogs but do not seem to influence gene transcription of IL‐4, IFN‐γ and TGF‐β.