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Role of hydroperoxide lyase in white‐backed planthopper ( Sogatella furcifera Horváth)‐induced resistance to bacterial blight in rice, Oryza sativa L.
Author(s) -
Gomi Kenji,
Satoh Masaru,
Ozawa Rika,
Shiaga Yumi,
Sanada Sachiyo,
Sasaki Katsutomo,
Matsumura Masaya,
Ohashi Yuko,
Kanno Hiroo,
Akimitsu Kazuya,
Takabayashi Junji
Publication year - 2010
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1111/j.1365-313x.2009.04031.x
Subject(s) - planthopper , infestation , oryza sativa , brown planthopper , biology , blight , xanthomonas oryzae , agronomy , horticulture , bacteria , botany , gene , hemiptera , biochemistry , genetics
Summary A pre‐infestation of the white‐backed planthopper (WBPH), Sogatella furcifera Horváth, conferred resistance to bacterial blight caused by Xanthomonas oryzae pv. oryzae ( Xoo ) in rice ( Oryza sativa L.) under both laboratory and field conditions. The infestation of another planthopper species, the brown planthopper (BPH) Nilaparvata lugens Stål, did not significantly reduce the incidence of bacterial blight symptoms. A large‐scale screening using a rice DNA microarray and quantitative RT‐PCR revealed that WBPH infestation caused the upregulation of more defence‐related genes than did BPH infestation. Hydroperoxide lyase 2 ( OsHPL2 ), an enzyme for producing C 6 volatiles, was upregulated by WBPH infestation, but not by BPH infestation. One C 6 volatile, ( E )‐2‐hexenal, accumulated in rice after WBPH infestation, but not after BPH infestation. A direct application of ( E )‐2‐hexenal to a liquid culture of Xoo inhibited the growth of the bacterium. Furthermore, a vapour treatment of rice plants with ( E )‐2‐hexenal induced resistance to bacterial blight. OsHPL2 ‐overexpressing transgenic rice plants exhibited increased resistance to bacterial blight. Based on these data, we conclude that OsHPL2 and its derived ( E )‐2‐hexenal play some role in WBPH‐induced resistance in rice.