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Capture of membrane components via trogocytosis occurs in vivo during both dendritic cells and target cells encounter by CD8 + T cells
Author(s) -
Riond J.,
Elhmouzi J.,
Hudrisier D.,
Gairin J. E.
Publication year - 2007
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.2007.01996.x
Subject(s) - cytotoxic t cell , microbiology and biotechnology , antigen presenting cell , biology , major histocompatibility complex , cd8 , antigen , cd40 , chemistry , in vitro , immunology , biochemistry
Abstract Cytotoxic T lymphocytes recently stimulated by antigen‐presenting cells (APC) display major histocompatibility class (MHC) I and II molecules inherited from APC. We have previously reported that, in vitro , transfer of MHC molecules and several other proteins occurs through trogocytosis, i.e. the active acquisition of target cell membrane fragments by T lymphocytes. Here, using the model of viral antigen LCMVgp33‐41 recognition in transgenic P14 mice, we show that CD8 + T cells perform trogocytosis in vivo , as detected by the capture of biotin‐ or fluorescence‐labeled components of the APC surface. Trogocytosis occurs during interactions of CD8 + T cells with at least two kinds of cells: target cells and dendritic cells (DC). In lymph nodes, CD8 + T cells having performed trogocytosis with DC express the CD69 activation marker indicating that trogocytosis detects recently activated cells. Taken together, our findings suggest that trogocytosis may be a new in vivo marker of the recent interaction between a CD8 + T cell and its cellular partners or targets.