Premium
Synchronous Deletion of Mtv‐Superantigen‐Reactive Thymocytes in the CD3 medium/high CD4 + CD8 + Subset
Author(s) -
Sheard M. A.,
Sharrow S. O.,
Takahama Y.
Publication year - 2000
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.2000.00814.x
Subject(s) - superantigen , cd8 , cd3 , microbiology and biotechnology , biology , major histocompatibility complex , t cell receptor , flow cytometry , antigen , thymocyte , clonal deletion , t cell , immunology , immune system
Multiple model systems have demonstrated that negatively selected thymocytes can be deleted during the immature CD4 + CD8 + CD3 low stage after high affinity interaction of T‐cell receptors (TCRs) with antigen:major histocompatibility complex (MHC) complexes. Superantigens (SAGs) derived from endogenous mammary tumour viruses (Mtv) induce negative selection of Mtv‐SAG‐reactive thymocytes regardless of which peptide antigen is presented by MHC molecules. In this study, the timing of deletion of multiple subsets of Mtv‐SAG‐reactive CD4 + CD8 + thymocytes was investigated by a 4 colour flow cytometry in SJL × CBA/J cross‐bred mice. Deletion of Vβ3 + , Vβ5 + , Vβ11 + , and Vβ17 + Mtv‐SAG‐reactive thymocytes was found to occur synchronously in the most mature CD3 medium and early CD3 high subsets of CD4 + CD8 + thymocytes, in contrast with reports showing that the deletion of Mtv‐SAG‐reactive thymocytes can occur at different stages in particular model systems.