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Measurement of Allergen‐Specific IgD and Correlation with Allergen‐Specific IgE
Author(s) -
ZHANG M.,
NIEHUS J.,
BRUNNÉE T.,
KLEINETEBBE J.,
O'CONNOR A.,
KUNKEL G.
Publication year - 1994
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1994.tb03496.x
Subject(s) - immunoglobulin d , allergen , immunoglobulin e , immunology , allergy , antibody , medicine , b cell
A new method for the measurement of allergen‐specific IgD (as‐IgD) was developed by modifying the ImmunoCAP assay (Pharmacia), and amplification of the signal with a goat anti‐human/rabbit antigoat detection system. The assay was sensitive enough to measure as‐IgD in serum samples. The specificity of the assay was examined using inhibition tests with excess corresponding and non‐corresponding allergens. For the different allergens inhibition rates between 56% (house dust mite) and 88% (cat) could be achieved. Non‐corresponding allergens did not inhibit the as‐IgD binding. Total IgE and allergen‐specific IgE (as‐IgE) was measured using the ImmunoCAP system. Total IgD was measured using a sandwich ELISA. As‐IgD was measured in serum samples from 51 atopic and 2.1 non‐atopic subjects, and the correlation with as‐IgE was examined. As‐IgD was detected in both atopies and non‐atopies but at higher levels in atopies. As‐IgD against birch pollen and timothy pollen allergen was found to be increased in atopies with IgE directed against these allergens compared to atopies without IgE against these allergens ( P <0.02 and P <0.03). As‐IgD against birch pollen allergen was higher in atopies with IgE specific to this allergen than in non‐atopies ( P <0.02). In contrast to total IgE and total IgD, significant correlations were observed between as‐IgD and as‐IgE against timothy pollen ( r = 0.34, P <0.04), birch pollen ( r = 0.38, P <0.05) and cat dander allergen ( r = 0.52, P <0.01). The observed correlations between as‐IgD and IgE suggest that IgD and IgE may be similarly regulated, and thus the measurement of as‐IgD may give further insight into the regulation of IgE.