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Different Conditions for Generation of Non‐Cytotoxic Autorestricted CD4 ‐ CD8 + Human Suppressor T Cells and Allospecific Cytotoxic T Lymphocytes in One‐Way Mixed Lymphocyte Cultures: the Role of Adherent Mononuclear Cells
Author(s) -
BALASHOV K. E.,
BRONDZ B. D.
Publication year - 1991
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1991.tb01590.x
Subject(s) - cytotoxic t cell , suppressor , cd8 , mixed lymphocyte reaction , t lymphocyte , peripheral blood mononuclear cell , cytotoxicity , immunology , microbiology and biotechnology , biology , chemistry , in vitro , t cell , immune system , biochemistry , gene
The purpose of the present study was to investigate the role of adherent mononuclear cells (AMC) in generation of suppressor T lymphocytes (T‐Lph) in one‐way allogeneic mixed lymphocyte cultures (M LC). Alloantigen‐specific cytotoxic T‐Lph were generated in MLC predominantly in the presence of stimulator AMC, but not responder AMC. The presence of responder AMC and the absence of stimulator AMC were optimal conditions for generation of non‐cytotoxic (through the whole duration of MLC) suppressor T‐Lph. These CD4 ‐ CDS8 + suppressor T‐Lph were allononspecific but autorestricted (with responders in test MLC).