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Quantification of the Terminal Complement Complex in Human Plasma by an Enzyme‐Linked Immunosorbent Assay Based on Monoclonal Antibodies against a Neoantigen of the Complex
Author(s) -
MOLLNES T. E.,
LEA T.,
FRØLAND S. S.,
HARBOE M.
Publication year - 1985
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1985.tb01871.x
Subject(s) - monoclonal antibody , antibody , complement system , in vivo , complement (music) , microbiology and biotechnology , monoclonal , enzyme , complement component 5 , chemistry , immunology , biology , biochemistry , phenotype , complementation , gene
The fluid‐phase terminal complement complex (TCC), consisting of the components C5b, C6, C7, C8, C9, and the S‐protein, has recently been detected in normal human plasma by using antibodies against native terminal complement components. Increased amounts of TCC were then found in several patients with in vivo activation of complement. We now describe a sensitive, specific, and reliable enzyme‐linked immunosorbent assay for quantification of the TCC, based on monoclonal antibodies against a neoantigen of the complex. The results indicate that the TCC is present in normal human plasma and in increased amounts in patients with complement activation in vivo, thus confirming previously obtained results. The assay is easy to perform and can be used for examination of large numbers of plasma samples.