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Isolation of Model Soluble Immune Complexes in the Fluid Phase by Monoclonal Anti‐IgG‐ Ferritin Antibodies
Author(s) -
KNUTSON D. W.,
RUDICK R. A.,
HERNDON R. M.
Publication year - 1985
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1985.tb01396.x
Subject(s) - monoclonal antibody , ferritin , immune complex , chemistry , dna , antibody , immunoglobulin g , immune system , microbiology and biotechnology , antigen antibody complex , biochemistry , biology , immunology
Ferritin conjugates of monoclonal IgG anti‐human gamma chain (anti‐IgG‐F) were reacted with soluble heat aggregates of IgG (A‐IgG) and with soluble DNA‐anti‐DNA complexes to increase the S rate of the model soluble immunecomplexes (IC x ) and thus facilitate isolation of IC x in the fluid phase and provide an immunochemical marker for subsequent ultrastructural analysis. A‐IgG appeared as globular or curvilinear structures withindividual IgG molecules arranged in a random fashion. The technique appears promising for characterization of other soluble IC x .