Premium
Mouse B‐ and T‐Cell Colony Formation in Vitro
Author(s) -
CLAESSON M. H.,
NISSEN M. HOLST,
ROPKE C.
Publication year - 1984
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1984.tb00921.x
Subject(s) - in vitro , sephadex , concanavalin a , spleen , microbiology and biotechnology , biology , chemistry , immunology , biochemistry , enzyme
Rat spleen coll cultures exposed for 24 h to concanavalin A (Con A‐CM) contain, in addition to inierleukin 2 (IL‐2). factors that promote colony formation in vitro by mouse T cells (TCPA) and B cells (BCPA). TCPA and BCPA are separable on a Sephadex G‐75 column. TCPA has a molecular weight of 15,000 daltons and shows the same elution profile as IL‐2. Absorption studies with Con A‐activated T cells suggested that TCPA and IL‐2 are the same entity. BCPA has an apparent molecular weight uf 45.000 daltons and stimulates colony formation by B lymphocytes seeded at very low cell density (10 4 –5 × 10 4 cells/ml). In contrast to TCPA, BCPA can only be demonstrated in gel‐filtered material owing to the presence of B colony suppressor activities in crude Con A‐CM. Two B colony inhibitory activities were demonstrated by AcA 34 chromatography of crude Con A—CM with molecular weights of 80,000–130.000 and about 50,000. respectively. Because of the specificity, simplicity and sensitivity of B and T colony formation these assay systems should be valuable tools for in vitro testing of biological activities regulating the immune system.