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Characterization of an Lyt‐l+ Cytolytic T‐Cell Clone Specific for a Polymorphic Domain of the I‐A k Molecule
Author(s) -
PIERRES A.,
SCHMITTVERHULST A.M.,
BUFERNE M.,
GOLSTEIN P.,
PIERRES M.
Publication year - 1982
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1982.tb00691.x
Subject(s) - clone (java method) , cytolysis , monoclonal antibody , microbiology and biotechnology , epitope , biology , antigen , lytic cycle , effector , antibody , t lymphocyte , t cell , virology , in vitro , cytotoxic t cell , immunology , biochemistry , immune system , gene , virus
We have characterized a cytolytic T‐cell clone, isolated from an A.TH anti‐A.TL mixed lymphocyte culture, which recognized a private determinant of the I‐A k molecule. This specificity has been confirmed by inhibition of effector‐target cell interaction by anti‐I‐A k monoclonal antibody (mAb). Comparison of the inhibitory capacity of various mAb and the spatial arrangement of their epitopes (defined in previous studies by antibody‐binding competition) indicated that the antigenic site recognized by this cytolytic T‐cell clone was topologically related to one of the major polymorphic domains of the A k molecule. This clone expressed the Thy‐1.2 + , Lyt‐1.2 + , Lyt‐2 + low and I‐A S ‐ cell surface phenotype. Testing of several rat mAb, screened for their ability to inhibit H‐2K/D ‐specific cytolysis at the level of the effector cells, revealed that two anti‐p94, 180 mAb but not various anti‐Lyt‐2 mAb inhibited the lytic function of this anti‐I‐A k T‐cell clone.