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Mouse Cell‐Mediated Lympholysis Assay in Serum‐Free and Mouse Serum‐Supplemented Media: Culture Conditions and Genetic Factors
Author(s) -
PECK A. B.,
BACH F. H.
Publication year - 1975
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1975.tb02599.x
Subject(s) - cytotoxic t cell , cytolysis , antigen , c57bl/6 , biology , microbiology and biotechnology , immunology , sensitization , cytotoxicity , chemistry , in vitro , genetics
We describe a miniaturized cell‐mediated lympholysis assay for measuring the activity of cytotoxic lymphocytes generated in mouse mixed leukocyte cultures (MLC), using either serum‐free or mouse serum‐supplemented media. Mixed reactions of strain combinations possessing various genetic differences at H‐2 produced cytotoxic lymphocytes with cytolytic activity against cells of the donor used in the sensitization phase. Results suggest that both serologically defined and lymphocyte‐defined determinants can act as effective target antigens on target cells. In contrast, no cytotoxic lymphocytes were generated against products of non‐H‐2 loci–M‐locus, theta, or other non‐H‐2 antigens–that induce MLC activation.