Premium
Delayed‐Type Hypersensitivity in the Mouse
Author(s) -
COOPER M.G.,
ADA G.L.
Publication year - 1972
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1972.tb01816.x
Subject(s) - biology , microbiology and biotechnology
The ability of thymus‐derived (T) cells activated by polymerized flagellin (POL) from Salmonella adelaide to transfer anti‐POL delayed‐type hypersensitivity (DTH) to normal mice was largely abrogated by pretreating the cells with anti‐Θ serum and complement, and completely abrogated by pretreating the cells with POL labelled with 125 I‐iodine to hight specific activity. The thymocyte precursors of POL‐activated T cells were also inactivated by treatment with 125 I‐POL. T cells activated by POL of one specificity were completely inactivated by 125 I‐POL of the same or of anothei specificity but not at all by 125 I‐labelIed haemocyanin (Jasus lalandii) . Preincubation of POL‐activated T cells with rabbit anti‐mouse light chain globulin prevented their subsequent inactivation by 125 I‐POL but did not reduce their ability to transfer anti‐POL DTH. The significance of these findings is discussed.