Premium
Eastern and southern African strains of Xanthomonas campestris pv. vasculorum are distinguishable by restriction fragment length polymorphism of DNA and polyacrylamide gel electrophoresis of membrane proteins
Author(s) -
QHOBELA M.,
CLAFLIN L. E.
Publication year - 1992
Publication title -
plant pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.928
H-Index - 85
eISSN - 1365-3059
pISSN - 0032-0862
DOI - 10.1111/j.1365-3059.1992.tb02327.x
Subject(s) - biology , xanthomonas campestris , restriction fragment length polymorphism , polyacrylamide gel electrophoresis , genetics , gel electrophoresis , pathogen , restriction fragment , genomic dna , population , genotype , microbiology and biotechnology , dna , bacteria , gene , biochemistry , demography , sociology , enzyme
Thirty strains of Xanthomonas campestris pv. vasculorum were examined using restriction fragment length polymorphism (RFLP) of genomic DNA and polyacrylamide gel electrophoresis (PAGE) of membrane proteins. Previous reports suggested the existence of two geographical strains of this pathogen, occurring primarily in eastern and southern Africa. Seventeen southern African strains from South Africa and Zimbabwe formed an apparent clonal population with distinct and unique RFLP patterns and membrane protein profiles. Similarly, 11 eastern African strains from Mauritius and Reunion and two from Australia were also related, indicating the possible existence of two distinct organisms separated by geographical locality but otherwise involved in the same disease. Our data confirm the long‐held hypothesis that eastern African strains of X. c. pv. vasculorurn are distinct from the southern African strains. The fact that these two distinct strains cause the same disease, gumming disease of sugar cane, suggests the possible independent development of the pathogen in these two localities.