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Use of forced buds to extend the period of serological testing in surveys for fruit tree viruses
Author(s) -
TORRANCE L.
Publication year - 1981
Publication title -
plant pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.928
H-Index - 85
eISSN - 1365-3059
pISSN - 0032-0862
DOI - 10.1111/j.1365-3059.1981.tb01259.x
Subject(s) - biology , prunus , sour cherry , horticulture , fruit tree , prunus cerasus , serology , botany , apple tree , cultivar , antibody , immunology
SUMMARY Forced buds were investigated as, a source of test material to determine whether they could be used to extend the period of reliable serological testing in routine surveys for some fruit‐tree viruses. Dormant bud‐sticks of cherry, plum and apple were cut in midwinter and either stored at 4°C or used immediately. Buds were forced in one to three weeks by keeping the budsticks at about 18°C. The emergent flower and leaf tissues were tested by enzyme‐linked immunosorbent assay for the presence of cherry leaf roll (CLRV), prune dwarf (PDV), strains of prunus necrotic ringspot (NRSV) and plum pox (PPV) viruses. PDV and the cherry serotype of NRSV (NRSV‐C) were detected in cherry and plum, CLRV in cherry, PPV in plum and the apple serotype of NRSV in plum and apple. Virus detection was not affected when cherry and plum budsticks were stored for 10 weeks at 4°C before testing. PDV, NRSV‐C and CLRV were still detected in cherry leaf and flower tissue wliich was forced and then stored at either 4 or ‐ 18°C for one to two months.