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Gene‐specific translational control of the yeast GCN4 gene by phosphorylation of eukaryotic initiation factor 2
Author(s) -
Hinnebusch Alan G.
Publication year - 1993
Publication title -
molecular microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.857
H-Index - 247
eISSN - 1365-2958
pISSN - 0950-382X
DOI - 10.1111/j.1365-2958.1993.tb01947.x
Subject(s) - biology , eif2 , initiation factor , ribosome , transfer rna , biochemistry , translation (biology) , gtp' , protein biosynthesis , eukaryotic translation , eukaryotic initiation factor , amino acid , gene , phosphorylation , microbiology and biotechnology , messenger rna , rna , enzyme
Summary Phosphorylation of the α subunit of eukaryotic initiation factor 2 (elF‐2α) is one of the best‐characterized mechanisms for down‐regulating total protein synthesis in mammalian cells in response to various stress conditions. Recent work indicates that regulation of the GCN4 gene of Saccharomyces cerevisiae by amino acid availability represents a gene‐specific case of translational control by phosphorylation of elF‐2α, Four short open reading frames in the leader of GCN4 mRNA (uORFs) restrict the flow of scanning ribosomes from the cap site to the GCN4 initiation codon. When amino acids are abundant, ribosomes translate the first uORF and reinitiate at one of the remaining uORFs in the leader, after which they dissociate from the mRNA. Under conditions of amino acid starvation, many ribosomes which have translated uORFI fail to reinitiate at uORFs 2‐4 and utilize the GCN4 start codon instead. Failure to reinitiate at uORFs 2‐4 in starved cells results from a reduction in the GTP‐bound form of elF‐2 that delivers charged initiator tRNA i Met to the ribosome. When the levels of elF‐2·GTP·Met‐tRNA i Met ternary complexes are low, many ribosomes will not rebind this critical initiation factor following translation of uORF1 until after scanning past uORF4, but before reaching GCN4. Phosphorylation of elF‐2 by the protein kinase GCN2 decreases the concentration of elF‐2·GTP·Met‐tRNA i Met complexes by inhibiting the guanine nucleotide exchange factor for elF‐2, which is the same mechanism utilized in mammalian cells to inhibit total protein synthesis by phosphorylation of elF‐2.