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A sandwich‐embedding method for oriented sectioning
Author(s) -
LEROUX O.,
VAN DER KINDEREN G.,
VIANE R. L. L.
Publication year - 2007
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.2007.01786.x
Subject(s) - embedding , overhead (engineering) , computer science , flexibility (engineering) , oblique case , transverse plane , economic shortage , materials science , artificial intelligence , engineering , structural engineering , mathematics , programming language , linguistics , government (linguistics) , statistics , philosophy
Summary High‐quality sections are indispensable for many scientific studies. Most published methods are often time‐consuming or require special devices. We present an easy, quick and low‐cost method for oriented embedding of thin structures using glycol methacrylate resin and self‐constructed, reusable embedding tools made of overhead transparencies. This technique allows for more flexibility in orientation than other methods, enabling precise transverse, longitudinal and even oblique sectioning.